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BIO462 Biochemistry UITM Assignment Sample Malaysia

BIO462 Biochemistry is an introductory course that focuses on the structures, functions, and reactions of biological molecules. The BIO462  course covers important biomolecules such as nucleic acids, carbohydrates, lipids, proteins, and enzymes. Students will also be introduced to metabolic pathways, including glycolysis, glycogenesis, gluconeogenesis, fermentation, respiration, lipid metabolism, and amino acid metabolism. Additionally, the course covers the processes of DNA transcription and translation. Overall, BIO462 Biochemistry provides a foundation for understanding the fundamental principles of biochemistry and its applications in biological systems.

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Assignment Brief 1 : Explain biomolecules with respect to their structure, function, biochemical reactions and characterization techniques in biochemistry

Biomolecules are essential molecules found in living organisms that play crucial roles in various biological processes.We  can be broadly classified into four main types: carbohydrates, lipids, proteins, and nucleic acids. Each biomolecule has a unique structure, function, and biochemical reactions associated with it. Additionally, several characterization techniques are employed in biochemistry to study and analyze biomolecules. In this assignment, you are required to explain the following aspects of biomolecules:


  • Describe the general structure of carbohydrates, lipids, proteins, and nucleic acids.
  • Discuss the different types and subtypes within each biomolecule category.
  • Explain the primary, secondary, tertiary, and quaternary structures of proteins.


  • Elaborate on the biological functions of carbohydrates, lipids, proteins, and nucleic acids.
  • Provide examples of how each biomolecule contributes to cellular processes and overall organismal function.

Biochemical Reactions:

  • Explain the metabolic reactions associated with carbohydrates, such as glycolysis and gluconeogenesis.
  • Describe the breakdown of lipids through processes like beta-oxidation.
  • Discuss protein synthesis, post-translational modifications, and enzymatic reactions.
  • Explain DNA replication, transcription, and translation in nucleic acids.

Characterization Techniques:

  • Highlight commonly used techniques such as spectroscopy (UV-Vis, IR, NMR), chromatography (HPLC, GC), and electrophoresis.
  • Explain how these techniques are employed to determine biomolecule structures, identify functional groups, and quantify biomolecules.

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Assignment Brief 2 : Display practical skills in biochemical experiments

In this assignment, you are required to demonstrate your practical skills in biochemical experiments. You can choose one or more experiments from the following areas:

Enzyme Activity:

  • Conduct an experiment to measure the activity of an enzyme by monitoring substrate conversion or product formation.
  • Determine the effect of temperature, pH, or enzyme concentration on enzyme activity.

Protein Analysis:

  • Perform protein extraction from a biological sample using appropriate techniques.
  • Utilize techniques such as SDS-PAGE or Western blotting to analyze protein size, abundance, or protein-protein interactions.

Nucleic Acid Analysis:

  • Extract DNA or RNA from a biological sample.
  • Perform polymerase chain reaction (PCR) to amplify specific DNA fragments or analyze gene expression.


  • Use UV-Vis spectroscopy to analyze the absorption or emission spectra of biomolecules.
  • Measure protein or nucleic acid concentrations using spectrophotometric techniques.

Ensure you carefully plan and execute your experiments, record observations, and analyze the results. Include detailed experimental procedures, materials used, data collected, and any relevant calculations or statistical analyses.

Assignment Brief 3 : Demonstrate communication skills in oral and written related to biochemistry

In this assignment, you will demonstrate your communication skills in oral and written formats, focusing on biochemistry topics. You can choose one or more of the following tasks:

Oral Presentation:

  • Prepare a presentation on a biochemistry-related topic of your choice, such as enzyme kinetics, protein structure, or DNA replication.
  • Clearly explain the chosen topic, including its background, significance, and key concepts.
  • Utilize visual aids, such as slides or diagrams, to enhance the presentation.
  • Deliver the presentation confidently, maintaining a clear and organized structure.

Written Report:

  • Write a comprehensive report on a recent biochemistry research paper or a specific biochemistry topic.
  • Summarize the key findings, methods used, and implications of the research or topic.
  • Provide a critical analysis of the strengths and limitations of the study or topic.
  • Use appropriate referencing and formatting guidelines.

Scientific Paper Review:

  • Choose a scientific paper from a biochemistry journal and critically review it.
  • Evaluate the paper’s experimental design, data analysis, and conclusions.
  • Discuss the significance of the findings and suggest areas for further research.
  • Clearly articulate your thoughts and support them with relevant evidence.

Ensure your communication is clear, concise, and well-structured. Pay attention to grammar, vocabulary, and effective use of scientific terminology. If applicable, provide references for the sources you consult during your task.

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