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BMS531 Methods In Molecular Biology I UITM Assignment Sample Malaysia

The BMS531 Methods in Molecular Biology I course at UITM introduces students to fundamental molecular biology techniques. Students will learn various hands-on techniques such as DNA extraction, gel electrophoresis, gene cloning, restriction enzyme mapping, DNA hybridization, DNA sequencing, and polymerase chain reaction. The course includes both lectures and tutorials to explain the mechanisms behind these techniques. By the end of the course, students will possess the necessary skills and understanding to delve into more advanced methods and comprehend the practical applications of molecular biology in research. Practical sessions will be conducted in a workshop-style format to maximize hands-on learning benefits.

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Assignment Activity 1 : Explain concepts relating to basic methods in molecular biology.

DNA Extraction: 

DNA extraction is the process of isolating DNA from cells or tissues. It is a fundamental step in molecular biology as DNA serves as the blueprint of life and is essential for many downstream applications.

Plasmid Extraction: 

Plasmid extraction involves isolating plasmid DNA from bacterial cells. Plasmids are small, circular pieces of DNA commonly used in genetic engineering and molecular cloning.

Agarose Gel Electrophoresis:

 Agarose gel electrophoresis is a technique used to separate DNA, RNA, or proteins based on their size and charge. It involves running a sample through an agarose gel in an electric field, causing the molecules to migrate and form distinct bands.

DNA Digestion and Ligation:

 DNA digestion uses restriction enzymes to cut DNA at specific recognition sequences, enabling the manipulation of DNA fragments. Ligation, on the other hand, is the process of joining DNA fragments together using DNA ligase.

Polymerase Chain Reaction (PCR):

 PCR is a powerful method used to amplify a specific region of DNA. It allows researchers to generate millions of copies of a target DNA sequence, enabling further analysis and experimentation.

Transformation: 

Transformation involves the introduction of foreign DNA (plasmids) into bacterial cells, making them capable of expressing new genes or traits.

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Assignment Activity 2 : Illustrate using specific examples the concepts relating to molecular biology of how it can be used to solve real problems.

Illustrating Molecular Biology Concepts with Examples:

Example 1: Genetic Engineering Molecular biology techniques are widely used in genetic engineering. For instance, by using restriction enzymes to cut a gene of interest from one organism and then ligating it into a plasmid, scientists can create recombinant DNA. This plasmid can be inserted into bacterial cells via transformation, allowing the bacteria to produce a specific protein encoded by the inserted gene.

Example 2: Diagnosing Genetic Diseases PCR is invaluable in diagnosing genetic diseases caused by specific mutations. Through PCR, even minute quantities of DNA with the mutation can be amplified for analysis, aiding in early detection and targeted treatment.

Assignment Activity 3 : Conduct experiments related to basic molecular biology techniques such as DNA/plasmid extraction, agarose gel electrophoresis, DNA digestion and ligation, PCR and transformation.

Experiments in Basic Molecular Biology Techniques:

  • DNA Extraction: Extract DNA from plant tissues or bacteria using a commercial DNA extraction kit or a homemade method involving grinding, cell lysis, and purification steps.
  • Agarose Gel Electrophoresis: Separate DNA fragments of known sizes using agarose gel electrophoresis and visualize the bands under UV light.
  • DNA Digestion and Ligation: Digest a plasmid and a DNA insert with appropriate restriction enzymes, purify the fragments, and ligate them together. Transform the ligated product into bacterial cells.
  • PCR: Perform a PCR reaction using primers specific to a target gene. Use genomic DNA or plasmid DNA containing the target gene as a template and amplify the gene of interest.
  • Transformation: Transform bacterial cells with a recombinant plasmid containing a fluorescent protein gene and observe the transformed colonies under UV light.

Assignment Activity 4 : Prepare scientific reports using data on experiments performed.

For each experiment, prepare a scientific report detailing the following:

  • Introduction: Provide background information and the purpose of the experiment.
  • Materials and Methods: Describe the materials used and the step-by-step methods employed.
  • Results: Present the data obtained from each experiment, including images or graphs.
  • Discussion: Analyze the results, explain any discrepancies, and discuss the significance of the findings.
  • Conclusion: Summarize the experiment’s outcomes and suggest potential areas for improvement.
  • References: Cite the sources of information and any scientific literature used in the report.

Remember to use proper scientific language and formatting throughout the reports.

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