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BCM202 Laboratory Methods In Biochemistry UITM Assignment Sample Malaysia

The course “BCM202 Laboratory Methods in Biochemistry” at UITM in Malaysia focuses on teaching students the fundamental techniques commonly used in biochemistry. The course covers various aspects, including pipetting techniques, buffer preparation, pH measurement, dilution calculations, and the use of spectrophotometry to determine concentrations.

Students will also be introduced to selected experiments that explore the properties of carbohydrates, lipids, and proteins. We  will learn essential biochemical techniques such as protein fractionation, protein electrophoresis, and the calculation of enzyme kinetic values.

To enhance learning, the BCM202  course incorporates laboratory demonstrations and presentations, ensuring that students acquire both knowledge and practical skills necessary to conduct experiments in biochemistry.

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Assignment Task 1 :Discuss the ability to conduct basic biochemical laboratory techniques in biochemistry lab practical

In a biochemistry lab practical, students learn and develop various basic biochemical laboratory techniques. These techniques are essential for conducting experiments and analyzing biological samples. Some of the commonly used techniques in a biochemistry lab practical include:

  • Pipetting: Pipetting involves the precise measurement and transfer of liquid samples. It is crucial for accurate preparation of reagents, dilutions, and sample handling.
  • Centrifugation: Centrifugation is used to separate components of a mixture based on their density and size. It is commonly employed to isolate cellular components, proteins, or DNA from a mixture.
  • Gel Electrophoresis: Gel electrophoresis is a technique used to separate and analyze macromolecules such as DNA, RNA, and proteins based on their size and charge. It involves the migration of molecules through a gel matrix under the influence of an electric field.
  • Spectrophotometry: Spectrophotometry measures the absorbance or transmission of light by a substance as a function of wavelength. It is widely used in biochemistry labs to determine the concentration of biomolecules and study enzymatic reactions.
  • Chromatography: Chromatography is a versatile technique used to separate and analyze mixtures. Common types of chromatography used in biochemistry labs include thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), and gas chromatography (GC).
  • Enzyme Assays: Biochemical labs often perform enzyme assays to measure the activity of enzymes and characterize their properties. These assays involve monitoring the conversion of a substrate into a product and quantifying the reaction rate.
  • Western Blotting: Western blotting is a technique used to detect and identify specific proteins in a sample. It involves the separation of proteins by gel electrophoresis, transfer to a membrane, and subsequent detection using specific antibodies.
  • PCR (Polymerase Chain Reaction): PCR is a technique used to amplify a specific DNA sequence. It is widely employed in molecular biology and biochemistry labs for DNA analysis, cloning, and gene expression studies.

These techniques are fundamental to biochemistry research and provide a solid foundation for more advanced experiments and investigations. Proficiency in these techniques enables students to accurately and reliably carry out experiments, collect data, and draw meaningful conclusions.

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Assignment Task 2 : Demonstrate the scientific investigations and data interpretation through verbally and in writing in biochemistry lab practical

In a biochemistry lab practical, students are expected to demonstrate their ability to conduct scientific investigations and interpret the resulting data. This involves the following steps:

  • Experimental Design: Students need to design experiments that address a specific research question or hypothesis. This includes selecting appropriate controls, experimental variables, and sample sizes.
  • Protocol Execution: Students should follow laboratory protocols meticulously, ensuring accurate and precise execution of experimental procedures. They need to record observations, measurements, and any modifications made during the experiment.
  • Data Collection: Accurate data collection is crucial in biochemistry lab practicals. Students should record their results, including measurements, observations, and any other relevant data points. This may involve using instruments, such as spectrophotometers or balances, and following specific measurement protocols.
  • Data Analysis: After collecting the data, students need to analyze it using appropriate statistical or computational methods. They may calculate averages, standard deviations, perform t-tests or ANOVA, and plot graphs to visualize their results.
  • Interpretation and Conclusion: Based on the data analysis, students should interpret their results and draw conclusions. They should discuss whether their findings support or refute their initial hypothesis and provide explanations for any unexpected observations.
  • Communication: Students should effectively communicate their findings verbally and in writing. This includes preparing lab reports, presentations, or posters that clearly outline the research question, methods, results, and conclusions. They should use scientific language, present data in a clear and organized manner, and provide appropriate references.

By demonstrating their scientific investigation skills and their ability to interpret data accurately, students in biochemistry lab practicals can showcase their understanding of experimental techniques, critical thinking, and scientific reasoning.

Assignment Task 3 :Express laboratory task on dealing with the method in biochemistry lab practical through literature

In biochemistry lab practicals, students often encounter various laboratory methods that are described in scientific literature. Expressing laboratory methods through literature involves understanding and effectively communicating the step-by-step procedures and techniques used in experiments. Here are some key points to consider:

  • Familiarity with Literature: Students should become familiar with relevant scientific literature, such as research articles, review papers, or laboratory manuals. These sources provide detailed descriptions of experimental methods, including the rationale behind each step and any variations or optimizations.
  • Reading and Comprehension: Students need to read the literature carefully, paying attention to the experimental procedures, reagents, equipment, and safety considerations mentioned in the text. They should ensure a clear understanding of the purpose and order of each step.
  • Note-taking and Summarizing: Taking detailed notes while reading the literature is crucial for accurately expressing laboratory methods. Students should summarize the key steps, reagents, and equipment required for each experiment. They can also create flowcharts or diagrams to visualize the experimental setup.
  • Clear and Concise Writing: When expressing laboratory methods in writing, students should aim for clarity and conciseness. They should use appropriate scientific terminology and describe each step in a logical and sequential manner. The use of bullet points or numbered lists can help in presenting the steps more clearly.
  • Illustrations and Figures: Including relevant illustrations or figures can enhance the clarity of the laboratory methods. Students can create diagrams, schematics, or flowcharts to illustrate the experimental setup or the procedural steps. This visual aid helps readers understand the experimental process more easily.
  • Referencing and Citations: It is important to provide proper references and citations when expressing laboratory methods through literature. Students should acknowledge the original source of the methods they are describing, ensuring proper credit to the authors. Following appropriate citation formats, such as APA or MLA, is essential.

By effectively expressing laboratory methods through literature, students demonstrate their ability to understand and communicate scientific techniques accurately. This skill is valuable for their own research endeavors, as well as for sharing knowledge and collaborating with the scientific community.

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