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BCM312 Laboratory Methods In Biochemistry UITM Assignment Answer Malaysia

BCM312 Laboratory Methods in Biochemistry at UITM Malaysia provides students with hands-on experience in essential techniques used in biochemistry laboratories. The course aims to familiarize students with various skills such as pipetting, buffer preparation, pH measurement, dilution calculations, and spectrophotometry for concentration determination. Students will also conduct experiments focusing on the properties of carbohydrates, lipids, and proteins. Additionally, the course emphasizes understanding biochemical techniques like protein fractionation, protein electrophoresis, and the ability to calculate enzyme kinetic values. By the end of the course, students will have gained practical knowledge and proficiency in fundamental biochemistry laboratory methods.

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Assignment Task 1 :Demonstrate the ability to conduct basic biochemical laboratory techniques

To demonstrate the ability to conduct basic biochemical laboratory techniques, we will focus on three common techniques: pipetting, gel electrophoresis, and spectrophotometry.

Pipetting:
Pipetting is a fundamental technique used to measure and transfer small volumes of liquids. Follow these steps to perform accurate pipetting:

  • Select the appropriate pipette size based on the volume to be transferred.
  • Attach a disposable pipette tip to the pipette.
  •   Depress the plunger to the first stop to draw the liquid into the pipette. 
  • Place the pipette tip into the receiving vessel and slowly release the plunger to dispense the liquid.

    Gel Electrophoresis:
      Gel electrophoresis is a technique used to separate and analyze DNA, RNA,       or proteins based on their size and charge. Here is a simplified protocol:

  • Prepare an agarose gel by dissolving agarose powder in buffer solution and heating it.
  • Pour the molten agarose into a gel tray and insert a comb to create wells for sample loading. 
  • Once the gel solidifies, remove the comb and place the gel in an electrophoresis chamber filled with buffer solution.
  •  Mix the samples with loading buffer, apply them to the wells, and run the gel at a suitable voltage for the desired time.
  •  After electrophoresis, visualize the separated bands using stains or dyes.

    Spectrophotometry:
    Spectrophotometry is a technique used to measure the absorbance or transmittance of light by a substance. Follow these steps to perform spectrophotometric analysis:

  • Prepare a sample solution of the substance to be analyzed. 
  •  Set up the spectrophotometer by adjusting the wavelength and blanking it with a reference solution or solvent.
  • Place the sample solution in a cuvette and insert it into the spectrophotometer.
  • Record the absorbance or transmittance reading, which indicates the concentration or properties of the substance.

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Assignment Task 2 :Verbally and in writing, discuss and report to peers the scientific investigations and data interpretation

When discussing and reporting scientific investigations and data interpretation to peers, it is important to communicate clearly and effectively. Here are some key points to consider:

  • Introduction: Provide a brief overview of the research question or objective of the investigation. Explain the significance of the study and its relevance to the field.
  • Materials and Methods: Describe the experimental setup, including the equipment and techniques used. Provide enough detail so that peers can understand and potentially replicate the experiment.
  • Results: Present the findings of the investigation in a clear and organized manner. Use tables, graphs, and figures to illustrate the data. Point out any trends, patterns, or significant observations.
  • Discussion: Interpret the results and discuss their implications. Compare your findings with existing literature or previous studies. Identify any limitations or sources of error in the experimental design. Offer possible explanations for unexpected results.
  • Conclusion: Summarize the key findings and their significance. Emphasize the main takeaways from the investigation and suggest future research directions.
  • Verbal Presentation: When presenting verbally, use a clear and confident tone. Organize your thoughts in a logical sequence and use visual aids, such as slides, to support your presentation. Engage your audience by encouraging questions and fostering discussion.
  • Written Report: In a written report, use a structured format with headings and subheadings. Ensure that your writing is concise, coherent, and free of grammatical errors. Cite relevant sources and include a list of references at the end of the report.

Remember, effective communication is key in scientific discussions and reporting. Tailor your message to your audience, use appropriate terminology, and focus on conveying the main points of your investigation and data interpretation.

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