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BMS557 Methods In Molecular Biology UITM Assignment Answer Malaysia

The “BMS557 Methods in Molecular Biology” course at UITM Malaysia introduces students to advanced techniques for manipulating biological molecules. Students will learn methods like gel electrophoresis, heterologous gene expression, mutagenesis, mutation detection, and fluorescence microscopy. Additionally, the course covers modern massively parallel analysis methods such as microarray and next-generation sequencing. The dynamic content of the course will be updated to reflect the latest developments in the field. The course is primarily hands-on, with lectures and tutorials to complement the practical training. By the end of the course, students will gain essential skills and understanding of molecular biology techniques and their research applications. The practical sessions will be conducted workshop-style to provide maximum hands-on learning benefits.

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Assignment Brief 1 : Describe the application of molecular biology techniques for gene analysis and manipulation.

Molecular biology techniques play a crucial role in the analysis and manipulation of genes, providing researchers with valuable tools to study the structure, function, and regulation of genes. These techniques have revolutionized the field of genetics and contributed significantly to various scientific and practical applications. Here are some key applications of molecular biology techniques for gene analysis and manipulation:

  • Polymerase Chain Reaction (PCR): PCR is a fundamental technique used to amplify specific DNA sequences. It allows researchers to produce millions of copies of a particular gene or DNA region rapidly. PCR is essential for various applications, such as DNA sequencing, gene cloning, and diagnostics (e.g., detecting genetic diseases and pathogens).
  • Gene Cloning: Molecular biology techniques enable the isolation and insertion of genes into vectors (e.g., plasmids) to create recombinant DNA. Cloning helps researchers study gene function, produce recombinant proteins, and engineer organisms with desired traits.
  • DNA Sequencing: DNA sequencing methods, such as Sanger sequencing and next-generation sequencing (NGS), provide the ability to determine the precise order of nucleotides in a DNA molecule. This information is invaluable for understanding gene structure and identifying genetic variations associated with diseases.
  • Gene Expression Analysis: Techniques like reverse transcription PCR (RT-PCR) and microarrays allow researchers to measure the expression levels of genes in different tissues or under varying conditions. This analysis helps to understand gene regulation and identify genes involved in specific biological processes.
  • Gene Editing: Molecular biology techniques like CRISPR-Cas9 have revolutionized gene editing. CRISPR enables precise modifications of DNA sequences, offering great potential for treating genetic diseases, engineering crops, and creating disease models for research.
  • RNA Interference (RNAi): RNAi is a technique used to silence specific genes by degrading their mRNA molecules. It has become a powerful tool for studying gene function and has potential therapeutic applications, particularly in treating diseases caused by malfunctioning genes.
  • Recombinant Protein Production: Molecular biology techniques are employed to express and produce large quantities of recombinant proteins, which are essential for biomedical research and the development of therapeutic drugs.

Assignment Brief 2 : Illustrate the principles and mechanisms underlying molecular biology methods e.g. gene analysis, sequence analysis and expression analysis; and how they can be applied to solve real world problems.

In Assignment Brief 2, you will explore the fundamental principles and mechanisms behind various molecular biology methods. Focus on explaining the following techniques and their applications:

  • Gene Analysis: Describe how techniques like PCR and gene cloning are used to analyze and study specific genes, understand their structure and function, and identify genetic variations associated with diseases.
  • Sequence Analysis: Explain the principles of DNA sequencing methods, such as Sanger sequencing and NGS. Highlight how sequence analysis helps in genome sequencing, identifying mutations, and understanding genetic diversity.
  • Expression Analysis: Illustrate the mechanisms of RT-PCR and microarray technology for measuring gene expression levels. Discuss how expression analysis aids in studying gene regulation and identifying genes involved in specific biological processes.
  • Gene Editing: Elaborate on the CRISPR-Cas9 system and its mechanism for precise gene editing. Showcase the potential applications of gene editing in treating genetic diseases and engineering organisms.
  • RNA Interference (RNAi): Describe the process of RNAi and how it can be used to silence specific genes to study their function and as a potential therapeutic approach for diseases.
  • Recombinant Protein Production: Explain the steps involved in expressing and producing recombinant proteins. Discuss the applications of recombinant proteins in biomedical research and drug development.

To illustrate real-world applications, you can provide examples of how these techniques have been used to solve problems in fields such as medicine (diagnostics and gene therapy), agriculture (genetically modified crops), and environmental science (genetic analysis of organisms).

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Assignment Brief 3 : Perform laboratory experiments employing basic molecular biology techniques

In Assignment Brief 3, you and your team will conduct laboratory experiments using fundamental molecular biology techniques. The objective is to gain hands-on experience with these techniques and apply them to real-world scenarios. The experiments may include:

  • PCR Amplification: Amplify a specific DNA fragment using the Polymerase Chain Reaction (PCR) technique. Choose a gene or DNA region of interest, design primers, and perform PCR to generate multiple copies of the target DNA.
  • DNA Gel Electrophoresis: After PCR, run the amplified DNA fragments on an agarose gel using gel electrophoresis. Visualize the DNA bands under UV light and analyze the results.
  • Plasmid DNA Isolation: Extract plasmid DNA from bacterial cells containing recombinant DNA constructs. Purify the plasmid DNA using common molecular biology protocols.
  • Gene Expression Analysis: Perform Reverse Transcription PCR (RT-PCR) to measure the expression levels of a specific gene in different tissue samples or experimental conditions.
  • Bacterial Transformation: Introduce a recombinant plasmid containing a gene of interest into bacterial cells and induce expression.

Assignment Brief 4 : Present written reports for laboratory experiments following good scientific writing format standards

In Assignment Brief 4, you will prepare written reports for the laboratory experiments conducted in Assignment Brief 3. Each team member should contribute to the report collaboratively. Follow these scientific writing format standards:

Provide a descriptive title that reflects the experiment’s objective.

Abstract: 

Summarize the experiment’s purpose, methods, key results, and conclusions in a concise paragraph.

Introduction: 

Describe the background and context of the experiment. Explain the significance of the techniques used and the relevance of the study.

Materials and Methods: 

Detail the experimental procedures with sufficient information so that others could replicate the experiment. Include reagents, equipment, and protocols used.

Results: 

Present the data obtained during the experiments in an organized manner. Use tables, graphs, and figures to illustrate the findings.

Discussion:

 Interpret the results and relate them to the experiment’s objectives. Analyze any discrepancies or unexpected observations. Compare your findings with published literature if applicable.

Conclusion: 

Summarize the main outcomes of the experiment and discuss their implications. Mention any limitations or areas for improvement.

References: 

Cite all sources and references used in the report.

Ensure the report is well-structured, coherent, and written in a clear and professional tone. Use appropriate scientific terminology and avoid jargon that may not be familiar to all readers.

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Assignment Brief 5 : Organize a team of peers to complete a laboratory task

In Assignment Brief 5, your role is to lead and organize a team of peers to complete a laboratory task related to molecular biology. Choose a task that aligns with the techniques and concepts covered in Assignment Briefs 3 and 4. The task could involve:

  • Designing and executing a group project to investigate the expression of specific genes in different cell lines.
  • Collaboratively planning and conducting a series of PCR experiments to amplify and analyze DNA samples from various sources.
  • Coordinating a gene editing project using CRISPR-Cas9 to target and modify specific genes in cell culture.
  • Organizing a team effort to analyze and interpret DNA sequence data using bioinformatics tools.
  • Working together on a project that explores the role of RNAi in gene silencing.

Clearly define the roles and responsibilities of each team member. Encourage open communication, cooperation, and regular progress updates. Set a timeline for completing the task and allocate resources effectively.

After the task is completed, your team will collectively prepare a presentation summarizing the project’s objectives, methods, results, and conclusions. Each team member should contribute to the presentation, which will be delivered to the class to showcase the successful teamwork and outcomes achieved in the laboratory task.

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