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CHM626 Organic Spectroscopy UITM Assignment Sample, Malaysia

CHM626 Organic Spectroscopy is a fundamental course offered at Universiti Teknologi MARA (UITM) designed to equip Malaysian students with the knowledge and skills necessary to analyze and determine the structures of organic compounds using various spectroscopic techniques. This course focuses on the practical applications of four main spectroscopic methods: Infrared Spectroscopy (IR), Nuclear Magnetic Resonance Spectroscopy (NMR), Ultraviolet-Visible Spectroscopy (UV-Vis), and Mass Spectrometry (MS), including hyphenated techniques.

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Assignment Activity 1: Explain the basic theory of IR, UV-Vis, 1H, 13C NMR and mass spectrometry and describe each of their applications in structure elucidation of simple organic compounds.

In the field of organic chemistry, several spectroscopic techniques are used for structure elucidation of simple organic compounds. These techniques include Infrared spectroscopy (IR), Ultraviolet-Visible spectroscopy (UV-Vis), Nuclear Magnetic Resonance spectroscopy (NMR), and Mass Spectrometry (MS). Let’s explore the basic theory of each of these techniques and their applications in structure elucidation:

Infrared Spectroscopy (IR):

  • Basic Theory: IR spectroscopy measures the vibrations of atoms within a molecule. When a molecule absorbs infrared radiation, it undergoes changes in vibrational and rotational energy. These changes are specific to the functional groups within the compound.
  • Applications: IR spectroscopy is useful for identifying functional groups in organic compounds. It can detect bonds like C=O, O-H, N-H, and C-H stretching vibrations. By analyzing the absorption peaks in an IR spectrum, chemists can deduce the presence of specific functional groups in a compound.

Ultraviolet-Visible Spectroscopy (UV-Vis):

  • Basic Theory: UV-Vis spectroscopy measures the absorption of ultraviolet and visible light by a compound. The energy differences between electronic energy levels within the molecule lead to distinct absorption patterns.
  • Applications: UV-Vis spectroscopy is mainly used for determining the presence of conjugated double bonds and chromophores in organic compounds. It is especially helpful in identifying compounds with extended π-electron systems, such as aromatic compounds.

Nuclear Magnetic Resonance Spectroscopy (NMR):

  • Basic Theory: NMR spectroscopy involves the interaction of atomic nuclei with a strong magnetic field. It measures the resonance frequencies of nuclear spins. For organic chemistry, 1H NMR measures the resonances of protons (hydrogen nuclei), and 13C NMR measures the resonances of carbon-13 nuclei.
  • Applications: NMR spectroscopy is a powerful tool for determining the connectivity of atoms in a molecule. It provides information about the number of unique hydrogen or carbon environments, their chemical environment (chemical shift), and their connectivity (coupling patterns). 1H NMR and 13C NMR are indispensable for deducing the complete structure of an organic compound.

Mass Spectrometry (MS):

  • Basic Theory: Mass spectrometry measures the mass-to-charge ratio of ions produced from a molecule. It involves ionization, fragmentation, and separation of ions based on their mass-to-charge ratios.
  • Applications: MS is essential for determining the molecular weight and identifying the molecular formula of an organic compound. It also provides information about the presence of specific functional groups and structural fragments through fragmentation patterns. MS can help in identifying the molecular structure of unknown compounds.

In summary, these spectroscopic techniques are invaluable for the structure elucidation of simple organic compounds. IR identifies functional groups, UV-Vis detects conjugated systems, NMR reveals atom connectivity, and MS provides information on molecular weight and fragmentation patterns. Together, these methods enable chemists to piece together the complete structure of organic molecules, aiding in research, quality control, and various applications in chemistry and related fields.

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Assignment Activity 2: Elucidate chemical structures of organic compounds from the interpretation of conjoint IR-UV-NMR-MS spectra.

Elucidating the chemical structure of an organic compound from the interpretation of conjoint IR (Infrared), UV (Ultraviolet), NMR (Nuclear Magnetic Resonance), and MS (Mass Spectrometry) spectra is a critical task in organic chemistry. Here’s a step-by-step guide to help you approach this:

Obtain the Molecular Formula: Begin by analyzing the MS (Mass Spectrometry) spectrum to determine the molecular formula of the compound. This data provides information about the compound’s molecular weight and composition in terms of the number of carbon, hydrogen, and other atoms.

Analyze the Mass Spectrum:

  • Look for the molecular ion peak (M+ or M+1), which represents the compound’s molecular weight.
  • Examine fragment peaks to identify characteristic structural fragments or functional groups that can provide clues about the compound’s structure.

Interpret the IR Spectrum:

  • Identify functional groups by analyzing the IR spectrum. Common absorptions include C=O, O-H, N-H, and C-H stretching vibrations.
  • Note any unique or distinctive peaks in the spectrum that may indicate the presence of specific functional groups.

Analyze the UV Spectrum:

Look for absorptions in the UV-Vis spectrum that suggest the presence of conjugated systems or chromophores. These can hint at the existence of aromatic rings or double bonds in the compound.

Examine the NMR Spectra:

  • Start with the 1H NMR (Proton Nuclear Magnetic Resonance) spectrum to determine the number of distinct hydrogen environments, their chemical shifts, and coupling patterns. This information helps identify the local environment of protons and the presence of neighboring functional groups.
  • Use the 13C NMR (Carbon-13 Nuclear Magnetic Resonance) spectrum to deduce the types of carbon environments and their connectivity within the compound.

Combine the Data:

  • Collaborate with your team or colleagues to integrate the information obtained from all the spectroscopic techniques.
  • Ensure that the proposed structure is consistent with all the data gathered. The structure must be compatible with the molecular formula, MS fragmentation patterns, IR functional group absorptions, and NMR chemical shifts and coupling information.

Iterate and Refine:

  • If the initial proposed structure doesn’t align with the spectral data, be prepared to revise and refine it.
  • Reevaluate the spectral data, considering alternative structures that better match the observed spectra.


  • Document your interpretations and findings for each spectroscopic technique to maintain a clear record of your analysis.

Elucidating the structure of an organic compound using these spectra is often a collaborative effort that may involve teamwork and communication with peers and colleagues. Sharing insights, discussing findings, and questioning inconsistencies are crucial in the process of structure determination.

The iterative nature of this process means that you may need to revisit your analysis, collaborate with others, and make adjustments to your proposed structure until it is fully consistent with all available spectral data

Assignment Activity 3: Elucidate structures of organic compounds from conjoint IR-UV-Vis-NMR-MS spectra Demonstrate communication skills in work collaboratively with peers and communicate effectively with instructor and peers on structure elucidation problems.

Elucidating the structures of organic compounds from conjoint IR (Infrared), UV-Vis (Ultraviolet-Visible), NMR (Nuclear Magnetic Resonance), and MS (Mass Spectrometry) spectra often requires effective communication and collaboration with peers and instructors. Here’s a guide to demonstrate strong communication skills while working together on structure elucidation problems:

Team Formation and Roles:

Collaborate with your peers to form a team for this assignment. Define roles for each team member based on their strengths, such as expertise in specific spectroscopic techniques (IR, UV, NMR, MS).

Information Sharing:

Establish clear channels of communication within the team. Regularly share data and insights through meetings, emails, or collaborative tools.

Data Analysis and Discussions:

  • Analyze the IR, UV-Vis, NMR, and MS spectra together, focusing on each team member’s area of expertise.
  • Hold discussions to extract important information from the spectra. For example, identify functional groups from IR, conjugated systems from UV-Vis, and carbon-hydrogen connectivity from NMR.

Instructor Engagement:

Maintain open and transparent communication with your instructor. Seek guidance when you encounter challenges or uncertainties in the structure elucidation process. Instructors can provide valuable insights.

Peer Reviews:

Regularly conduct peer reviews to challenge and validate each other’s interpretations. Constructively critique proposed structures and spectral analyses, encouraging discussion and refinement.


Keep detailed records of your findings, including spectral data, proposed structures, and the rationale behind your decisions. Use a shared platform to centralize this information for easy reference by the team.

Feedback and Revision:

Encourage constructive feedback within the team. If a proposed structure doesn’t align with the spectra or is questioned by a team member, revise it and discuss the changes.

Iterative Process:

Understand that structure elucidation is often an iterative process. Be open to revisiting your analysis and making necessary adjustments as you gain more insights and information.

Effective Presentations:

If required, prepare a clear and concise presentation to communicate your findings and proposed structure to your peers and instructor. Use visual aids to support your explanations.

Conflict Resolution:

In the case of disagreements or conflicts within the team, approach these situations with professionalism and a focus on finding the best solution for the group’s success.

Timely Collaboration:

Meet deadlines and milestones for your collaborative work. Effective time management and communication are key to the success of the assignment.

Respect and Encouragement:

Foster a positive and respectful team environment where every member’s contributions are valued. Encourage and support each other’s efforts.

Demonstrating strong communication skills and collaborative teamwork is essential for successful structure elucidation from conjoint spectroscopic data. Effective interactions with peers and instructors help ensure that the proposed structure is well-informed and robust, making the assignment a productive and valuable learning experience.

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